Heparin sodium is a natural anticoagulant of acid mucopolysaccharide containing sulfate group. In the blood collection tube additives, mainly used for blood anticoagulation, by inhibiting the activity of thrombin to prevent blood coagulation. Those who know know that heparin sodium is purchased and used according to its potency. Therefore, it is necessary to understand the factors that influence its potency, and the high value of heparin itself is also to avoid unnecessary losses.
Effect of dissolution temperature on dissolution time and titer of heparin sodium
With the increase of temperature, the titer of heparin sodium increased firstly and then decreased, and reached the maximum value at 40℃. If the temperature is too low, crude heparin will take longer to dissolve, resulting in a waste of production costs. If the temperature is too high, the activity of heparin sodium will be destroyed, resulting in a sharp decrease in the titer of heparin sodium. Considering the titer of heparin sodium and the cost of dissolving time, the optimal dissolving temperature is 40℃.
Effect of salt concentration on the titer of heparin sodium
Protein can be separated from heparin in an appropriate concentration of salt solution, but when salt concentration is too low, the impurity protein cannot be completely separated from heparin, thus affecting the activity of heparin. When the salt concentration is too high, the high concentration of salt solution will destroy the structure of heparin, leading to the reduction of heparin activity. The titer of heparin sodium reached the highest value when the concentration of sodium chloride reached 0.35mol/L.
Effect of thermal denaturation time on the titer of heparin sodium
With the prolonging of thermal denaturation time, the titer of heparin sodium increased first and then decreased, which might be caused by the gradual denaturation of the impurity protein with the prolonging of time. If the time is too long, the structure of heparin is destroyed at high temperature, which affects the activity of heparin and leads to the gradual decrease of potency. The optimum time for thermal denaturation is 10 minutes.
Effect of hydrogen peroxide dosage on the titer of heparin sodium
Excessive hydrogen peroxide destroys the molecular structure of heparin sodium and partially inactivates the heparin sodium, leading to an increase and then a decrease in potency. Therefore, when the volume ratio of hydrogen peroxide to solution volume is 1%, it is the optimal amount of hydrogen peroxide.
Qingdao Jiulong Medicine reminds you as a Heparins Manufacturer:
Do not use crude products directly, use refined heparin sodium, the appearance of the color is pink and white.
Heparin sodium dissolved in water can be directly sprayed on the wall of blood collection tube, which can play an anticoagulant effect.
Conventional dose of 1 ml of blood corresponds to 20IU of heparin sodium, but excessive dose cannot achieve the best anticoagulant effect.
Storage method of preparation solution: low temperature 0-4℃. Since heparin sodium cannot be stored for a long time after preparation, it should be prepared now and stored for a maximum of 7 days.