Heparin Sodium API is mainly derived from the intestinal mucosa of pigs, cows and sheep. However, heparin steel derived from cattle and sheep has the risk of infection by related viruses, and the probability of adverse reactions such as thrombocytopenia and thrombotic syndrome is much higher than that of pig heparin steel. Therefore, people should choose porcine heparin sodium as far as possible in clinical use. However, in actual production, the production materials from pigs may be contaminated by animal materials from cattle, sheep and other sources due to the complex sources of production materials and other reasons. Therefore, it is very important to establish identification methods for animal derived ingredients such as pig, sheep and cattle to control drug quality and prevent contamination of heterogeneous animal derived ingredients.
Heparin Sodium API Supplier Introduces the detection principle of Heparin sodium from different sources:
Heparin sodium is a glycosaminoglycan composed of glucuronic acid (L. iduronic acid, IDOAD-glucuronic acid, GlcA) and glucosamine (6[. D-glucosamine, GlcN), which is a mixture of polysaccharide chains with different chain lengths. Different kinds of heparin sodium were hydrolyzed by heparin enzyme and their hydrolysates were analyzed. That is, the species origin of the product is characterized by the composition of the enzymolysis product (disaccharide mixture).
Heparinase is a polysaccharide lyase that acts on heparin or heparin sulfate molecules. There are three types of heparinase, namely, heparinase I, heparinase II and heparinase III. Because each type of heparin enzyme has a unique matrix specificity, it can cleft specific sites in the heparin chain. Heparin 1 dissolves the 0C (1-4) glycosidic bond between GlcNs(6S) and IdoA(2S), thus cutting off the antithrombin III binding site in the heparin molecule. Heparinase can decompose heparin containing 2 or 3 sulfates in disaccharides, so its specificity is not strong. Heparin II1 decomposes heparin with 6 unsulfonated GIcNS or Topper. Acetyl glucosamine (GlcNAc). An appropriate mixture of the three heparin enzymes can completely degrade heparin into an unsaturated disaccharide mixture with uv absorption at 234nm.
Heparin sodium API source tests are mainly used to identify different types of heparin sodium by quantitative PCR, but this method is expensive. Heparin enzymolysis method can effectively identify heparin samples mixed with sheep crude heparin, and it is simple and rapid. The only disadvantage of heparinase is that it loses the information of the differential isomer of glucuronic acid and iduronic acid during the enzymatic hydrolysis of heparin. In addition, in recent years, capillary electrophoresis has been used to separate various disaccharides. Heparin disaccharide can also be separated by reversed-phase ion pair chromatography and quantified by mass spectrometry.