Heparin is called heparin because it was originally found in the liver and is a natural anticoagulant found in many mammalian organs, most of which are found in the lungs and intestinal mucosa. Common heparin is a kind of aminoglucosan sulfate extracted and refined from pig intestinal mucosa or bovine lung. It is a mixture with a molecular weight range of 3000-30000KD and an average of about 15000KD. The aminoglucan sulfate fragment after pyrolysis of common heparin, i.e., low molecular weight heparin heparin calcium, enoxaparin, daheparin, etc., has an average molecular weight range of 3000-8000KD.
Preparation of crude heparin
The fresh pig intestines (or frozen pig intestines after natural thawing) are carefully cleaned and treated with water, and then enzymolysis is carried out: the raw materials are fully stirred, finely adjusted to PH value of 8-9 with a small amount of dilute lye, and the liquid temperature is kept at 37-40 degrees for enzymolysis for 3-4 hours. Refined salt (containing NaCL≥95% and calcium magnesium calcium salt <0.5%) of the total weight of the material liquid was added to make the mixture evenly miscible, then the temperature was raised 90 degrees, the heat was kept for 30 minutes, and the filtrate was adjusted to PH=9.0-9.5 for ion exchange adsorption treatment.
Elution, filtration, combination of clear liquid and filtrate, fine adjustment of PH=6.0-6.5, add 1.5 times the amount of 95% ethanol precipitation overnight, the next day, carefully drying out the supernatant, collect the lower precipitate, drain to dry (mother liquid can be used in the eluent before PH adjustment). It is dried in a vacuum, and the result is a crude heparin.
Heparin sodium finished product refining
The crude heparin was completely dissolved in 2% sodium chloride solution to produce a solution with a solubility of about 8%. In this process, the temperature could be raised appropriately to help dissolve.
Use 5mol/L sodium hydroxide solution to fine-adjust PH=8.0-8.2 at night, heat up to 78-80 degrees, add 0.15-0.2mol/L potassium permanganate solution for oxidation, fine filtration, filter the material liquid with "bactericidal filter", add 3-4 times 95% ethanol for precipitation, acetone dehydration and fine grinding, Far infrared vacuum drying (50-60 degrees), ready to heparin sodium boutique.