At present, heparin sodium is mainly extracted from the small intestine mucosa of pigs and sheep and the lung of cattle. Studies have shown that the content of heparin sodium is the highest in the small intestine mucosa of pigs. Crude heparin sodium is our traditional export product and occupies an important position in the world.
Heparin sodium can interfere with many parts of the blood clotting process and has anticoagulant effects both in vivo and in vitro. There are many methods for the extraction of heparin sodium. The following describes the commonly used extraction process of heparin sodium.
1.the small intestine is washed, and then the mucosa of the small intestine is scraped out and mashed. Spare.
2.the mud heated water stir, and then add trypsin and weak alkali in turn, until the solution ph value of 8-10, and then heating, heating process ph should be kept between 8.5-9.5, heating to 30-40℃, maintain a constant temperature of 2-3h, and then continue to heat to 50-60℃, maintain a constant temperature of 10-20min, Then do deep filtration treatment, get filtrate;
3. Cool the filtrate to 30-40℃, remove the oil slick on the upper layer of the filtrate, and then add A-722MP resin column to absorb heparin sodium;
4. add 10% sodium chloride solution, keep the temperature at 50-55℃, slowly elute.
5. Filter and transfer the eluent collected twice into the precipitation tank, add 80-85% alcohol and stir evenly, then adjust the ph value to keep at 7-8, let the sealed precipitation stand for 10-12 hours, filter and get the sediment.
6. put the sediment into the drying box after dehydration and drying to obtain heparin sodium.