Heparin is a mucopolysaccharide widely existing in mammalian tissues, mainly in mast cells, with anticoagulant effect. It is widely used in surgery and the treatment of cerebral thrombosis and myocardial infarction. The molecular weight of heparin is about 3000-37500. Studies of commercially available heparin show that there are at least 21 individual molecules. Heparin sodium is the sodium salt of heparin. As a kind of natural anticoagulant substance, it has been paid attention to all over the world. It is also one of China's main export drugs.
With the deepening of research, it is found that it not only has anticoagulant, antithrombotic, lipid regulation, but also has anti-inflammatory, anti-allergy, anti-virus, anti-cancer and other functions. At present, it is mainly extracted from the mucous membrane of small intestine of pig and sheep, bovine lung, villous casing brine and so on. Studies have shown that it is present in high levels in the intestinal mucosa of pigs. Crude oil products are China's traditional export products and occupy an important position in the world.
However, the traditional production process needs to heat up to 50-55℃ for 2 hours, and then add 3_4% sodium chloride to heat up to 85-95℃, which can overcome the problems of short holding time and incomplete enzymatic hydrolysis. For every I kg of coal mined, 520-580 kg of coal is needed, so a lot of energy is consumed, and coal burning is more serious to the environment.
Implementation: Pour water into the small intestine of pigs, and the intestinal mucosa is extruded to the mucous membrane pool by the casing machine; The casing handle is wound to form the casing handle, and then the casing handle is soaked in the soaking solution for 4.5 hours, removed and drained, the casing handle is cured with special salt for casing, and then the casing handle is soaked in the soaking solution for 7 hours, the first soaking solution is saturated sodium chloride solution, the second soaking solution is sodium chloride solution with salinity of 16 degrees; During the first soaking and the second soaking, 160 casings per 1000 liters of soaking solution were soaked, and the first soaking solution was collected for reserve, and the second soaking solution was collected as the first soaking solution for the next extraction;
The pig intestine mucosal slurry is added to the enzymatic hydrolysis tank, and the pig intestine mucosal slurry is a mixture with the weight ratio of pig intestine mucosa and water of 1∶3. The collected soaking extract is added to the enzymatic hydrolysis tank, and the salinity of the feed liquid in the enzymatic hydrolysis tank is adjusted to 5 degrees, and the PH value of the feed liquid is controlled to 8.5. Then add 2709 alkaline protease to the enzymolysis tank according to the amount of 1.2kg of enzyme per 1000L feed liquid, control the feed liquid temperature 53℃, salinity 4.2, PH 8.5, for 10 hours; Then raise the temperature to 63℃.