Crude heparin and heparin sodium product distinction introduction!

  Heparin, because it was first found in the liver, is a natural anticoagulant found in many mammalian organs, with lung and intestinal mucosa being the most abundant. The current heparin is a kind of sulfurated aminopolysaccharide extracted from pig intestine mucosa or bovine lung. It is a mixture with molecular weight ranging from 3000 to 3000-30000kD, with an average of about 15000KD. The average molecular weight of aminopolysaccharides sulfate fragments after the cutting of ordinary heparin, namely low molecular weight heparin calcium, enoxaparin, daheparin sodium, etc., is 3000-8000kD.

  Preparation of crude heparin

  After the fresh pig intestines (or frozen pig intestines thawed naturally) are carefully cleaned with water, enzymolysis is carried out: under full stirring, the PH value of the raw material is fine-tuned to 8-9 with a small amount of dilute lye, and the liquid temperature is kept at 37-40 degrees. Enzymolysis is carried out for 3-4 hours. Add about 5% refined salt (containing NaCL≥95%, calcium magnesium calcium salt)

Crude heparin and heparin sodium product distinction introduction!

  Elution, extraction and filtration, combined supernatant and filtrate, fine adjust PH=6.0-6.5, add 1.5 times of 95% ethanol precipitation overnight, dry carefully the next day, suck out the supernatant, collect the lower sediment, extraction and filtration to dry (mother liquor can be used for eluent before adjusting PH). After vacuum drying, crude heparin was prepared.

  Purification of heparin sodium products

  Crude heparin was completely dissolved in 2% sodium chloride solution to prepare a solution with a solubility of about 8%. In the process, the temperature can be raised to help dissolve.

  Use 5mol/L sodium hydroxide solution to fine-tune the pH value of the above feed solution to 8.0-8.2, heat up to 78-80 degrees, add 0.15-0.2mol/L potassium permanganate solution oxidation, fine filtration, feed liquid filtration with "sterilizing filter", coupled with 3-4 times of 95% ethanol precipitation, acetone dehydration, grinding, Far infrared vacuum drying (50-60 degrees), heparin sodium boutique.

  As can be seen from the above, crude Heparin is the raw material, which can be further refined to get the finished Heparin sodium. Jiulong Pharmaceutical uses Heparins API Manufacturer and Heparin Sodium API supplier, which is of good quality and affordable.