The heparin sodium is extracted from the mucosal membrane of the small intestine of pigs. The heparin solution is lost after enzymatic hydrolysis by 2709 protease, and then the egg white in the mucosal membrane of the small intestine of pigs is agglutinated by sodium chloride and the temperature is lowered, so that the natural heparin sodium can easily exchange ions. The strong basic ion exchange resin after washing with dilute sodium chloride solution was eluted with high concentration of sodium chloride solution, and the eluted heparin sodium was accumulated in alcohol solution and dried to produce crude heparin sodium.
The crude heparin sodium was acidified to remove the acid hybrid egg white, and then refined by two oxidation processes. The unit titer of the product was more than 140 ~ 150 international units per mg, and the product was sterile and pyrogen-free, especially in the preparation process, there was no wastewater infection environment.
Heparins Manufacturer to introduce：Specific steps: put the recycled resin into clean water to soak, remove and filter dry; The presence proportion of the filtered resin is soaked in salt water of 23 degrees, and the filtrate is filtered out. The presence of salt water is 0.7 times that of the resin. The filtrate is soaked in salt water with a specific gravity of 25 degrees, and the filtrate is filtered. The amount of salt water is 0.7 times that of resin. Filtrate A and filtrate B were mixed with ethanol of 85 degrees until the filtrate alcohol was 35 degrees, and the red precipitate was heparin sodium. Because of the use of water purification immersion, the resin is fully expanded, and the acceptance of two different concentrations of salt water immersion, so that the adsorption of heparin sodium in the resin is fully eluted, not only increased the amount of heparin sodium extraction, but also improved the resin adsorption capacity.