1, the fresh pig lung (or frozen pig lung after natural thawing) with water after fine cleaning to remove the internal and external dirt and external skin fat, ground into minced, and in full stirring, adding the same amount of water mixed, then add a little solubility of.01% preservative mixed evenly.
2, enzymatic hydrolysis extraction: first the above raw materials in full stirring, with a small amount of dilute lye fine adjustment to the PH value of 8-9. Then add fresh minced pancreatic pulp as the enzyme hydrolysant, stir well, and slowly heat up to about 40 degrees Celsius.
Continue stirring, and maintain the liquid PH=7.5-8, 37-40 degrees, enzymatic hydrolysis for 3-4 hours, then heat up to 47-50 degrees, maintain PH= 8.0-8.5, and then add a little pig pancreatic juice, and continue the enzymatic hydrolysis for 4-5 hours.
3, ion exchange adsorption; Firstly, the enzyme hydrolysate was cooled to room temperature, and the thin layer of oil floating on the liquid level was carefully removed. The temperature was controlled to 45 degrees, and the heating was stopped. After stirring, the heparin sodium in the material liquid which had been effectively adsorbed by d-254 resin pretreated was added.
4. Refining: The crude heparin sodium obtained is completely dissolved with 2% sodium chloride solution to make a solution with a solubility of about 8%. In this process, the temperature can be appropriately raised to help the solution. The above material was heated to 78-80 degrees by using 5mol/L sodium hydroxide solution at night to finely adjust the PH=8.0-8.2.
The precipitate is dehydrated by anhydrous ethanol, finely ground, and then dehydrated by acetone, finely ground, and then dehydrated by acetone, far infrared vacuum drying (50-60 degrees), to obtain heparin sodium fine.
Heparins Manufacturer: For the prevention and treatment of thrombotic or embolic diseases such as myocardial infarction, thrombophlebitis, pulmonary embolism, etc. Diffuse intravascular coagulation (DIC) caused by various causes; Also used in hemodialysis, extracorporeal circulation, catheterization, microvascular surgery and other operations and some blood specimens or instruments anticoagulation treatment. Want to know Heparin sodium price, welcome to consult understand!