Heparin sodium is a natural anticoagulant of acid mucopolysaccharide containing sulfuric acid group. It is mainly used for blood anticoagulation in blood vessel additives to prevent blood clotting by inhibiting thrombin activity. Heparins Manufacturer introduces that Heparin sodium is purchased and used according to its potency, and Heparin sodium price is a common concern. Let's learn about the factors that affect Heparin sodium potency.
Effect of dissolution temperature on dissolution time and therapeutic effect of heparin sodium
The titer of heparin sodium increased firstly and then decreased with the increasing of ambient temperature, and the titer of heparin sodium reached a maximum at 40℃. If the temperature is too low, the dissolution time of crude heparin will be longer, resulting in the waste of production and management cost of enterprises. The temperature can not be too high, it will destroy the activity of heparin sodium, resulting in a sharp increase in the titer of heparin sodium. Considering the potency, reaction time and cost of heparin sodium, the optimal dissolution temperature was 40℃.
Effect of salt concentration on the titer of heparin sodium
Appropriate concentration of salt solution processing allows the separation of the protein and heparin, but when the salt concentration is too low, the impurity binding protein separation with heparin is not complete, so as to affect the use of heparin activity, when the salt concentration is too high, high concentration of salt solution can destroy, molecular heparin with heparin biological activity decreased significantly. When the concentration of sodium chloride did not reach 0.35mol/L, the titer of heparin sodium reached a maximum value.
Effect of thermal denaturation time on the titer of heparin sodium
With the prolongation of heat denaturation time, the titer of heparin sodium increased first and then decreased, which may be because the impurity protein in the process of heat denaturation gradually degenerated with the prolongation of time. If the time is too long, the structure of heparin will be destroyed at high temperature, thus affecting the activity of heparin and leading to the gradual decrease of titer. The best time for thermal denaturation is 10 minutes.
Effect of different amounts of hydrogen peroxide on the titer of heparin sodium
Excessive hydrogen peroxide will damage the molecular structure of heparin sodium, resulting in partial inactivation of heparin sodium, leading to the first rise and then fall of the titer.
Heparins Manufacturer reminds you:
Instead of using crude products, use refined heparin, which is powdered and white in color. Heparin sodium is dissolved in water and made into a solvent, which can be sprayed directly on the wall of blood collection tube to achieve anticoagulation effect. The conventional material dosage is 1mL of blood corresponding to 20IU of heparin sodium, too much can not fail to achieve the best teaching effect of anticoagulation.
Solution storage method: Store at a low temperature of 0-4 degrees. Because heparin cannot be prepared after long-term storage, it should be prepared immediately, and the maximum storage period should not exceed 7 days. The above is the effect of Heparin sodium potency and matters needing attention, want to understand Heparin sodium price welcome to Kowloon for consultation!