Let's look at the specific process with Heparins Manufacturer.
1. Fresh pig lungs (or frozen pig lungs thawed naturally) are carefully washed with water to remove internal and external dirt and skin fat, and then minced into minced meat. After stirring thoroughly, add equal amount of water and mix, then add a small amount of 0.01% solubility preservative and mix well.
2, enzymatic extraction: first through the above main raw materials in full use of stirring, with a small amount of dilute lye fine adjustment to the pH value of 8-9. Then add fresh pancreatic pulp which can be designed in advance and crushed as enzymatic hydrolyzing agent. After stirring well, the temperature is raised slowly to about 40 degrees.
Continue stirring, keep the liquid pH =7.5-8, keep the liquid temperature 37-40 degrees, enzymatic hydrolysis for 3-4 hours, then heat to 47-50 degrees, keep the pH =8.0-8.5, then add a small amount of porcine pancreatic plasma, continue enzymatic hydrolysis for 4-5 hours.
3. Ion exchange adsorption. First, cool the enzyme extract to room temperature, carefully remove the oil sheet layer floating on the surface of the liquid, control the heating to 45 degrees, stop the heating, and add the pretreated D-254 resin under stirring which has effectively adsorbed the content of liquid heparin sodium.
4. Refining: the crude heparin sodium is completely dissolved in 2% sodium chloride solution to get a solution with solubility of about 8%. Carefully adjust the above substances in 5mol/ L sodium hydroxide solution to pH = 8.0-8.2 and temperature to 78-80 degrees.
The precipitate was dehydrated with anhydrous ethanol, ground, dehydrated with acetone, ground, dehydrated with acetone, and vacuum dried with far infrared (50-60℃) to obtain refined heparin sodium. If you want to know about Heparin Sodium Price, please come to consult!